Original line was established by Tak Mak at Ontario Cancer Institute in Tronto (Schmits et al., J. Exp. Med., 183, 1415, 1996). Neo registance gene construct was introduced into the CD11a allele of an ES cell line E14k of 129/Ola origin. Mutant ES cells were injected to C57BL/6 blastocysts. Chimeras were mated with C57BL/6 and was backcrossed to C57BL/6 for 6 generations. After embryo transfer at Taconic, the colony has been maintained through intercrossing of homozygous mice.
Virology Research
RBRC00850
慶應義塾大学
条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>利用者はnon-profit organizationを対象とし、Dr. Tak W. Makの許可を得ること。<br>開発者連絡先:Tak W. Mak, Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute E-mail: tmak@uhnresearch.ca
herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, tk poly A, mouse CD11a genomic DNA
From Tak. W. Mak of ontario Cancer Institute, N6
From Tak. W. Mak of ontario Cancer Institute, N6
C57BL/6-[KO]LFA-1; B6.129P2-Itgb2<tm1Oci>
C57BL/6-[KO]LFA-1; B6.129P2-Itgb2<tm1Oci>
E14K [129P2/OlaHsd]
Shigeo KOYASU
Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www.ims.riken.jp/labo/43/index.html" target="_blank">Lab HP</A>
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER (Tak W. Mak, Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute E-mail: tmak@uhnresearch.ca).
B6.129P2-Itgaltm1Mak
Original line was established by Tak Mak at Ontario Cancer Institute in Tronto (Schmits et al., J. Exp. Med., 183, 1415, 1996). Neo registance gene construct was introduced into the CD11a allele of an ES cell line E14k of 129/Ola origin. Mutant ES cells were injected to C57BL/6 blastocysts. Chimeras were mated with C57BL/6 and was backcrossed to C57BL/6 for 6 generations. After embryo transfer at Taconic, the colony has been maintained through intercrossing of homozygous mice.
Original line was established by Tak Mak at Ontario Cancer Institute in Tronto (Schmits et al., J. Exp. Med., 183, 1415, 1996). Neo registance gene construct was introduced into the CD11a allele of an ES cell line E14k of 129/Ola origin. Mutant ES cells were injected to C57BL/6 blastocysts. Chimeras were mated with C57BL/6 and was backcrossed to C57BL/6 for 6 generations. After embryo transfer at Taconic, the colony has been maintained through intercrossing of homozygous mice.
C(3〜6か月)
covid19_infection
C (3-6 months)
Immunology and Inflammation Research
C57BL/6-[KO]LFA-1; B6.129P2-Itgb2tm1Oci
C57BL/6-[KO]LFA-1; B6.129P2-Itgb2tm1Oci
<a href='https://brc.riken.jp/mus/pcr00850'>Genotyping protocol -PCR-</a>
小安 重夫
B6.129P2-Itgal<tm1Mak>
Keio University
true